2024
T. Hatakeyama, K. Masuda, M. Kudo, K. Tanaka, A. Takeuchi, H. Unno. “Mannose
oligosaccharide recognition of CGL1, a mannose-specific lectin containing
DM9 motifs from Crassostrea gigas, revealed by X-ray crystallographic analysis” J. Biochem., 175(1), 35-41
(2024)
T. Hatakeyama, F. Kojima, I. Ohkawachi, H. Sawai, H. Unno. "Carbohydrate-binding ability of a recombinant protein containing the DM9 motif from Drosophila melanogaster" J. Biochem., in press (2024)
2023
T. Hatakeyama and H. Unno. “Functional Diversity of Novel Lectins with Unique Structural Features in Marine Animals” Cells, 12(14), 1814 (2023)
2021
T. Hatakeyama, A. Kishigawa, H. Unno. "Molecular cloning and characterization
of the two putative toxins expressed in the venom of the devil stinger
Inimicus japonicus” Toxicon, 201, 9-20 (2021)
M. Okada, H. Unno, K. Emi, M. Matsumoto, and H. Hemmi. "A versatile
cis-prenyltransferase from Methanosarcina mazei catalyzes both C- and O-prenylations" J. Biol. Chem., 296, 100679
(2021)
M. Kawakita, T. Oyama, I. Shirai, S. Tanaka, K. Akaki, S. Abe, T. Asahi, G. Cui, F. Itoh, M. Sasaki, N. Shibata, K. Ikuta, T. Hatakeyama, K. Takahara "Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis" Communications Biology, 4(342), 1-9 (2021)
Y. Liang, M., S. Zha, T. Okimura, Z. Jiang, K. Yamaguchi, T. Hatakeyama, and T. Oda. “Sulfated polysaccharide ascophyllan prevents amyloid fibril formation of human insulin and inhibits amyloid-induced hemolysis and cytotoxicity in PC12 cells” Biosci. Biotechnol. Biochem., 85(11), 2281-2291 (2021)
2020
K. Imamura, T. Matsuura, A. Nakagawa, S. Kitamura, M. Kusunoki, T. Takaha, H. Unno.
"Structural analysis and reaction mechanism of the disproportionating enzyme (D-enzyme) from potato" Protein Science. 29(10), 2085-2100 (2020)
Novel carbohydrate-recognition mode of the invertebrate C-type lectin SPL-1 from Saxidomus purpuratus revealed by the GlcNAc-complex crystal in the presence of Ca2+,
Acta Crystallogr. F Struct. Biol., Commun. 76(6), 271-277（2020）
H. Unno, S. Higuchi, S. Goda, T. Hatakeyama.
Lectin Purification and Analysis : Methods and Protocols (Methods in Molecular Biology (2132), 1st ed. 2020 Edition, Editors: Hirabayashi J., Publisher: Springer US,
DOI: 10.1007/978-1-0716-0430-4, Hardcover ISBN: 978-1-07-160429-8
https://www.springer.com/gp/book/9781071604298#aboutBook
Chapter 16, Galactose-Specific, Hemolytic Lectin CEL-III from Cucumaria
echinate
Tomomitsu Hatakeyama
Chapter 53, Mannose-Specific Oyster Lectin CGL1
Hideaki Unno and Tomomitsu Hatakeyama
Chapter 56, Purification of AJLec, a Novel Galactose-Specific Lectin, from
the Sea Anemone Anthopleura japonica
Hideaki Unno
2019
H. Unno, S. Itakura, S. Higuchi, S. Goda, K. Yamaguchi, and T. Hatakeyama"Novel Ca2+-independent carbohydrate recognition of the C-type lectins,
SPL-1 and SPL-2, from the bivalve Saxidomus purpuratus" Protein Science.
28 (4) , 776-778 (2019.4)
2018
S. Goda, T. Koga, K. Yamashita, R. Kuriura, T. Ueda "A novel carbohydrate-binding surface layer protein from the hyperthermophilic archaeon Pyrococcus horikoshii" Biosci.Biotechnol. Biochem. 82 (8), 1327-1334 (2018.8)
H. Unno, A. Nakamura, S. Mori, S. Goda, K.
Yamaguchi, K. Hiemori, H. Tateno, T. Hatakeyama "Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Lectin AJLec from the Sea Anemone Anthopleura japonica"
Sci. Rep. 8, 11516 (2018.8)
2017
T. Hatakeyama, A. Ichise, H. Unno, S. Goda, T. Oda, H. Tateno, J. Hirabayashi,
H. Sakai, H. Nakagawa "Carbohydrate recognition by the rhamnose-binding
lectin SUL-I with a novel three-domain structure isolated from the venom
of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus"
Protein Sci. 26 (8) 1574-1583. (2017.5)
K Motoyama, H Unno, A Hattori, T Takaoka, H Ishikita, H Kawaide, T Yoshimura, H Hemmi "A Single Amino Acid Mutation Converts (R)-5-Diphosphomevalonate Decarboxylase into a Kinase" J. Biol. Chem. 292 (6) 2457-2469 (2017.2)
2016
T. Nagao, R. Masaki, H. Unno, S. Goda, T. Hatakeyama "Effects of amino acid mutations in the pore-forming domain of the hemolytic lectin CEL-III" Biosci. Biotechnol. Biochem. 80 (10), 1966-1969 (2016.10)
H. Unno, K. Matsuyama, Y. Tsuji, S. Goda, K. Hiemori, H. Tateno, J. Hirabayashi, T. Hatakeyama "Identification, characterization, and X-ray crystallographic analysis of a novel type of mannose-specific lectin CGL1 from the pacific oyster Crassostrea gigas " Sci. Rep. 6, article number 29135 (2016.7)
T. Hatakeyama, S. Goda, H. Unno "Mechanism of Action of the Pore-Forming Lectins Mediated by Binding to Cell Surface Carbohydrate Chains" Trends Glycosci. Glycotechnol. 28 (161), E55-E60 (2016.5)
2015
無脊椎動物由来溶血性レクチンの細胞膜孔形成機構 CEL-Ⅲによる膜孔形成メカニズム, 海野 英昭, 化学と生物, 53(2), 74-75 (2015)
T. Hatakeyama, E. Higashi, H. Nakagawa "cDNA cloning and expression of Contractin A, a phospholipase A2-like protein from the globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus" Toxicon 108, 46-52 (2015.12)
A Hattori, H Unno, S Goda, K Motoyama,T Yoshimura, H Hemmi "In Vivo Formation of the Protein Disulfide Bond That Enhances the Thermostability of Diphosphomevalonate Decarboxylase, an Intracellular Enzyme from the Hyperthermophilic Archaeon Sulfolobus solfataricus" J. Bacteriol. 197 (21), 3463-3471 (2015.11)
H. Moriuchi, H. Unno, S. Goda, H. Tateno, J. Hirabayashi, T. Hatakeyama "Mannose-recognition mutant of the galactose/N-acetylgalactosamine-specific C-type lectin CEL-I engineered by site-directed mutagenesis" Biochim. Biophys. Acta 1850 (7) 1457-1465 (2015.7)
T. Hatakeyama, A. Ichise, T. Yonekura, H. Unno, S. Goda, and H. Nakagawa "cDNA cloning and characterization of a rhamnose-binding lectin SUL-I from the toxopneustid sea urchin Toxopneustes pileolus venom" Toxicon 94, 8-15 (2015.2)
2014
1. Y. Kato, K. Kochi, H. Unno, S. Goda, T. Hatakeyama "Manno-oligosaccharide-binding ability of mouse RegIV/GST-fusion protein evaluated by complex formation with the carbohydrate-containing polyamidoamine dendrimer" Biosci. Biotechnol. Biochem. 78 (11) 1906-1909 ( 2014.11)
2. K Ohara, H Unno, Y Oshima, M Hosoya, N Fujino, K Hirooka, S Takahashi, S Yamashita, M Kusunoki, and T Nakayama "Structural Insights into the Low-pH Adaptation of a Unique Carboxylesterase from Ferroplasma: Altering the pH Optima of Two Carboxylesterases" J. Biol. Chem. 289 (35), 24499-24510 (2014.8)
3. H. Unno, S. Goda, and T. Hatakeyama "Hemolytic lectin CEL-III heptamerizes via a large structural transition from alpha-helices to beta-barrel during the transmembrane pore-formation process" J. Biol. Chem. 289 (18) 12805-12812 (2014.5)
4. S. Kudo, J. M. M. Caaveiro, S. Goda, S. Nagatoishi, K. Ishii, T. Matsuura, Y. Sudou, T. Kodama, T. Hamakubo, and K. Tsumoto "Identification and characterization of the X-dimer of human P-cadherin: Implications for homophilic cell adhesion" Biochemistry 53 (11), 1742-1752 (2014.3)
2013
1. H. Unno, K. Hisamatsu, T. Nagao, Y. Tateya, N. Matsumoto, S. Goda, and T. Hatakeyama “Crystallization and preliminary crystallographic study of oligomers of the hemolytic lectin CEL-III from a sea cucumber Cucumaria echinata” Acta Cryst. F69, 416-420 (2013.3)
2. S. Goda, H. Sadakata, H. Unno, and T. Hatakeyama “Effects of detergents on the oligomeric structures of the hemolytic lectin CEL-III as determined by small-angle X-ray scattering” Biosci. Biotechnol. Biochem. 77 (3), 679-681 (2013.3)
3. T. Hatakeyama , T. Ishimine, T. Baba, M. Kimura, H. Unno and S. Goda “Alteration of the Carbohydrate-Binding Specificity of a C-type Lectin CEL-I Mutant with an EPN Carbohydrate-Binding Motif” Protein Pept. Lett., 20 (7), 796-801 (2013.7)
4. K. Hisamatsu, T. Nagao, H. Unno, S. Goda, T. Hatakeyama “Identification of the amino acid residues involved in the hemolytic activity of the Cucumaria echinata lectin CEL-III” Biochim. Biophys. Acta, 1830, 4211-4217 (2013.4)
5. M. Ueno, T. Yamanishi, T. Hatakeyama, K. Yamaguchi, T. Oda “Effects of lycopene on the secretion of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) from RAW264.7 cells stimulated with marine invertebrate Holothuroidea (Cucumaria echinata) lectin CEL-I” Jpn. J. Food Chem. Safety, 20 (3), 196-202 (2013.12)
6. The catalytic Role of Reduced Flavin Mononucleotide in Type 2 Isopentenyl Diphosphate Isomerase, Hisashi Hemmi and Hideaki Unno, Flavins and Flavoproteins 2011, pp. 33-41, Editor: Susan Miller, Russ Hille, Bruce Palfey Publisher: Lulu.com (2013), ISBN-10: 130078640X, ISBN-13: 978-1300786405
2012
1. T. Hatakeyama, R. Karino, Y. Terai, M. Kimura, H. Unno, S. Goda “An assay for Carbohydrate-Binding Activity of Lectins using Polyamidoamine Dendrimer Conjugated with Carbohydrates” Biosci. Biotechnol. Biochem. 76 (10) 1999-2001 (2012.10)
2. S. Kudo, J. M. M. Caaveiro, T. Miyafusa, S. Goda, K. Ishii, T. Matsuura, Y. Sudou, T. Kodama, T. Hamakubo, K. Tsumoto “Structural and thermodynamic characterization of the self-adhesive properties of human P-cadherin” Molecular Biosyst. 8 (8) 2050-2053 (2012.08)
3. H. Nakatani, S. Goda, H. Unno, T. Nagai, T. Yoshimura, H. Hemmi, “Substrate-induced change in the quaternary structure of type 2 isopentenyl diphosphate isomerase from Sulfolobus shibatae”, Journal of Bacteriology 194 (12), 3216–3224 (2012.06)
2011
1. Tomomitsu Hatakeyama, Takuro Kamiya, Masami Kusunoki, Sachiko Nakamura-Tsuruta, Jun Hirabayashi, Shuichiro Goda, and Hideaki Unno “Galactose recognition by a tetrameric C-type lectin, CEL-IV, containing the EPN carbohydrate-recognition motif” J. Biol. Chem., 286(12), 10305-10315 (2011)
2. 畠山智充：はじめて学ぶ生命科学の基礎（畠山智充，小田達也編著），1，4，8，9章，化学同人（2011）
3. 郷田秀一郎：はじめて学ぶ生命科学の基礎（畠山智充，小田達也編著），4，5，9章，化学同人（2011）
4. T. Nagai, H. Unno, M. W. Janczak, T. Yoshimura, C. D. Poulter, H. Hemmi, "Covalent modification of reduced flavin mononucleotide in type 2 isopentenyl diphosphate isomerase by active-site-directed inhibitors" Proc. Natl. Acad. Sci. USA, Vol. 108, No. 51, pp. 20461-20466 (2011)
5. C. Zwieb, Y. Nakao, T. Nakashima, H. Takagi, S. Goda, E. S.Andersen, Y. Kakuta, M. Kimura, “Structural modeling of RNase P RNA of the hyperthermophilic archaeon Pyrococcus horikoshii OT3”, Biochem. Biophys. Res. Commun., Vol. 414, pp. 517-522 (2011)
2010
1. Masayoshi Hatayama, Hideaki Unno, Masami Kusunoki, Seiji Takahashi, Tokuzo Nishino and Toru Nakayama “Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases (AmCHS1)” J. Biosci. Bioeng., 110(2), 158-164 (2010)
2. Shu Jie Li, Qing Zhao, Qiangjun Zhou, Hideaki Unno, Yujia Zhai, Fei Sun, “The role and structure of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1.”, J. Biol. Chem. 285(16), 12047-12054 (2010)
3. Norihisa Yasui, Emiko Mihara, Maiko Nampo, Keiko Tamura-Kawakami, Hideaki Unno, Kyoichi Matsumoto, Junichi Takagi, “Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies speci?c for the native conformation”, Journal of Immunological Methods,352, 153-160 (2010)
4. Zedong Jiang,Daekyung Kim, Yasuhiro Yamasaki, Tomohiro Yamanishi, Tomomitsu Hatakeyama, Kenichi Yamaguchi, and Tatsuya Oda “Mitogenic activity of CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, isolated from the marine invertebrate Cucumaria echinata (Holothuroidea)”,Biosci. Biotechnol. Biochem., 74(8), 1613-1616 (2010)
5. Haruhiko Aoyagi, Akira Hirano, Tomomitsu Hatakeyama, Setsuko Ando, Taizo Kita, and Masato Asanuma, A study on bioactivity and function of chemical compounds in food and foodstuff: Interaction of linear and dendritic cationic peptides with phospholipid membranes and their antibacterial activity, 7号, 1-11 (2010)
2009
1. Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda and Tomomitsu Hatakeyama,"Roles of the Valine Clusters in Domain 3 of the Hemolytic Lectin CEL-III in Its Oligomerization and Hemolytic Abilities", Protein Pept. Lett., 16(4), 411-414 (2009)
2. 畠山智充, "海産無脊椎動物由来溶血性レクチンの糖認識と溶血活性発現機構" , 生物物理 Vol. 49, 075-079 (2009)
3. Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda, and Tomomitsu Hatakeyama,"Effects of Ca2+ on Refolding of the Recombinant Hemolytic Lectin CEL-III", Biosci. Biotechnol. Biochem., 73(5), 1203-1205 (2009)
4. Hideaki Unno, Satoshi Yamashita, Yosuke Ikeda, Shin-ya Sekiguchi, Norie Yoshida, Tohru Yoshimura, Masami Kusunoki, Toru Nakayama, Tokuzo Nishino, and Hisashi Hemmi, “New Role of Flavin as a General Acid-Base Catalyst with No Redox Function in Type 2 Isopentenyl Diphosphate Isomerase”, J. Biol. Chem. 284(14), 9160-9167. (2009)
5. Tetsuo Yamashita, Yoshio Moria, Naoyuki Miyazaki, R. Holland Cheng, Masato Yoshimura, Hideaki Unno, Ryoichi Shima, Kohji Moriishi, Tomitake Tsukihara, Tian Cheng Li, Naokazu Takeda, Tatsuo Miyamura and Yoshiharu Matsuura, “Biological and immunological characteristics of hepatitis E virus-like particles based on the crystal structure.”, Proc. Natl. Acad. Sci. U. S. A. 106(31), 12986-12991 (2009)
6. Tomohiro Yamanishi, Tomomitsu Hatakeyama, Kenichi Yamaguchi, and Tatsuya Oda, “CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, induces nitric oxide production in RAW264.7 mouse macrophage cell line”, J. Biochem., 146(2), 209-217 (2009)
7. Ryushi Kawakami, Haruhiko Sakuraba, Shuichiro Goda, Hideaki Tsuge and Toshihisa Ohshima, “Refolding, characterization and crystal structure of (S)-malate dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix”, Biochimica et Biophysica Acta 1794:1496-1504 (2009)
8. 郷田秀一郎、櫻庭春彦、大島敏久, “大腸菌で生産される超好熱菌由来の上活性型グルタミン酸脱水素酵素の活性化機構”, 生化学, 81, 1049-1055 (2009)
2008
1. Keigo Hisamatsu, Nobuaki Tsuda, Shuichiro Goda and Tomomitsu Hatakeyama, "Characterization of the alpha-helix region in domain 3 of the hemolytic lectin CEL-III: Implications for self-oligomerization and hemolytic processes", J. Biochem., 143 (1), 79-86 (2008)
2. Tetsuo Yamashita, Hideaki Unno, Yoshio Mori, Hideki Tani, Kohji Moriishi, Akihisa Takamizawa, Masanobu Agoh, Tomitake Tsukihara and Yoshiharu Matsuura, "Crystal structure of the catalytic domain of Japanese encephalitis virus NS3 helicase/nucleoside triphosphatase at a resolution of 1.8 Å", Virology 373(2):426-436 (2008)
3. Junichi Taira, Sachi Furukawa, Tomomitsu Hatakeyama, Haruhiko Aoyagi and Hiroaki Kodama, "Modifications of hydrophobic value and hydrophobic moment value of cationic model peptides for conversion of peptide-membrane interactions", Bull. Chem. Soc Jpn., 81(6), 733-739 (2008)
4. Nobuaki Tsuda, Yukari Koba, Tomomitsu Hatakeyama, Setsuko Ando, Mitsuno Shindo, Yoshiki Uchida and Haruhiko Aoyagi, "Antibacterial Activity of Short Peptides Based on Pleurocidin and their Interaction with Phospholipid Membranes", Bull. Chem. Soc Jpn., 81(10), 1299?1303 (2008)
5. Tomomitsu Hatakeyama, "Hemolytic Lectin in Marine Invertebrates", Animal Lectins (G.R. Vasta and H. Ahmed, eds.), pp, 517-529, CRC Press (2008)
6. Hideaki Unno, Tetsuo Yamashita, Sayuri Ujita, Nobuaki Okumura, Hiroto Otani, Akiko Okumura, Katsuya Nagai, and Masami Kusunoki, "Structural basis for substrate recognition and hydrolysis by mouse carnosinase CN2", J. Biol. Chem. 283(40), 27289-27299 (2008)
7. Yoshikazu Tanaka, Sou Sakamoto, Makoto Kuroda, Shuichiro Goda, Yong-Gui Gao, Kouhei Tsumoto, Yuzuru Hiragi, Min Yao, Nobuhisa Watanabe, Toshiko Ohta, Isao Tanaka, "A Helical String of Alternately Connected Two Three-helix Bundles for the 1.1-Megadalton Cell Wall-Associated Adhesion Protein Ebh from Staphylococcus aureus", Structure 16(3), 188-196 (2008)
8. 郷田秀一郎、櫻庭春彦、柊弓絃、大島敏久, “大腸菌体内で上活性型遺伝子組換え酵素として生産される好熱菌由来のグルタミン酸脱水素酵素の活性化とそれに伴う構造変化”, ビタミン, 82, 337-343 (2008)
2007
1. 畠山智充， "動的光散乱法によるタンパクｿ結合反応の解析", バイオプロセスハンドブック -バイオケミカルエンジニアリングの基礎から有用物質生産・環境調和技術まで-, （株）エヌ・ティー・エス, pp. 348-355 (2007)
2. 畠山智充，"レクチン-糖質間相互作用の定量的解析法"，生物化学実験法52「レクチン研究法《，山崎信行他編著，（株）学会出版センター，pp. 79-99 （2007）
3. Hideaki Unno, Fumiko Ichimaida, Hirokazu Suzuki, Seiji Takahashi, Yoshikazu Tanaka, Atsushi Saito, Tokuzo Nishino, Masami Kusunoki, and Toru Nakayama, "Structural and Mutational Studies of Anthocyanin Malonyltransferases Establish the Features of BAHD Enzyme Catalysis",J. Biol. Chem. 282 (21), 15812-15822 (2007)
4. Nicolas Rouhier,, Hideaki Unno, Sibali Bandyopadhyay, Lluis Masip, Sung-Kun Kim, Masakazu Hirasawa, Jose Manuel Gualberto, Virginie Lattard, Masami Kusunoki, David B. Knaff, George Georgiou, Toshiharu Hase, Michael K. Johnson, and Jean-Pierre Jacquot, "Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1", Proc. Natl. Acad. Sci. U. S. A. 104 (18), 7379-7384 (2007)
5. Tomohiro Yamanishi, Yoshiko Yamamoto, Tomomitsu Hatakeyama, Kenichi Yamaguchi, and Tatsuya Oda, "CEL-I, an Invertebrate N-Acetylgalactosamine-Specific C-Type Lectin, Induces TNF-alpha and G-CSF Production by Mouse Macrophage Cell Line RAW264.7 Cells", J. Biochem. 142 (5), 587-595 (2007)
6. Tomomitsu Hatakeyama, Hideaki Unno, Yoshiaki Kouzuma, Tatsuya Uchida, Seiichiro Eto, Haruki Hidemura, Norihisa Kato, Masami Yonekura and Masami Kusunoki, "C-Type lectin-like carbohydrate-recognition of the hemolytic lectin CEL-III containing ricin-type beta-trefoil folds",J. Biol. Chem. 282 (52), 37826-37835 (2007)
7. 中山亨, 田中良和, 海野英昭, 楠木正巳,第4章 産業の発展に貢献できるタンパク質４.１”花色改変技術への期待：アントシアニンアシル基転移酵素の機能と構造” ,構造生物学—ポストゲノム時代のタンパク質研究—, 倉光成紀・杉山政則編, 共立出版, pp. 159-169 (2007)
8. Wataru Saburi, Hironori Hondoh, Hideaki Unno, Masayuki Okuyama, Haruhide Mori, Toshitaka Nakada, Yoshiki Matsuura and Atsuo Kimura, "Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase", Acta Crystallographica Section F Structural Biology and Crystallization Communication F63 774-776, (2007)
9. Toshihisa Ohshima, Ryushi Kawakami, Yuichiro Kanai, Shuichiro Goda, Haruhiko Sakuraba, "Gene expression and characterization of 2-keto-3-deoxygluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of the aerobic and acidophilic hyperthermophile Sulfolobus tokodaii", Protein Expression and Purification 54(1), 73-78 (2007)